Authentic Biochemistry
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You have wisely landed upon Authentic Biochemistry, with Dr. Daniel J Guerra, where published biochemical, physiological, genetic and immunological research along with biomedical and pharmaceutical science are interrogated.
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Episodes
The Innate Immune Response Instantiates the Pattern for future Inflammatory Prodromal Neurodegeneration. DJGPhD. 08May 2021
May 08, 2021 19:19 - 30 minutes - 27.5 MBPathogens, and abiotic ischaemic damage manifest a constellation of molecular interactions triggering pattern recognition receptors such as TLR's, with canonical protein binding domains that signal through intracellular cascades such as activating Ca2+ signaling; eo ipso obtaining potent hyperimmune inflammation, providing event ontological foundation and episodic contribution to the aging morbidity in human decline and mortality. --- Support this podcast: https://podcasters.spotify.c...
Pattern Recognition Receptors: Some molecular detail. DJGPhD Authentic Biochemistry. 07 May 2021
May 07, 2021 22:40 - 29 minutes - 27 MBOncotarget. 2016 Jan 26; 7(4): 4195–4209 Nature Reviews Immunology volume 6, pages644–658(2006) --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Ischemic Infarct as a model for immune mediated CNS regeneration or degeneration via microglial stimulation. Dr. Daniel J Guerra. Authentic Biochemistry . 06May2021
May 06, 2021 18:06 - 29 minutes - 27.4 MBThe removal of damaged neurons and associated synaptic connections with the simultaneous repair of other neuronal circuits is a mechanism for the sculpting of the brain network and thus a means by which the highly innervated and connected prefrontal cortex can receive and respond to neurotransmission from the limbic system. Reference: Genes (Basel). 2019 Mar; 10(3): 249 --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Lymphocyte and Resident Microglia participate in CNS formation, Epigenetic Modifications and Neurodegeneration via RAG-Recombinase. Authentic Biochemistry Podcast by DJGPhD. 27 April 2021
April 28, 2021 00:53 - 29 minutes - 27.3 MBAuthentic Biochemistry Podcast 27 April 2021 References Clin Diagn Lab Immunol. 2003 Jan;10(1):13-8 J Exp Med (2012) 209 (1): 187–199. Cell Reports 2020. V 30, (Issue 5) :1585-1597.e6 --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
The RAG Recombinase Interacts with T lymphocyte Programmed Cell Death Loci. Dr Dan Guerra Authentic Biochemistry Podcast 26 April 2021
April 26, 2021 18:45 - 29 minutes - 27.3 MBReferences Experimental & Molecular Medicine volume51, Article number: 80 (2019) Am J Nephrol.2017;46(5):371-379 EMBO Rep . 2003 Aug;4(8):774-80 J Biol Chem. 2020 Jul 3;295(27):9052-9060 Mol Immunol. 2017Aug;88:125-134 --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Dr. Guerra presents: The CNS and the Immune System are linked to Recombination and the Epigenome. Lecture IV. 24.4.21 Authentic Biochemistry
April 24, 2021 18:33 - 29 minutes - 27.3 MBAs an hypothetico-deduction, Dr. Guerra proposes that the Recombination Activating Gene-RAG System is a Potential Candidate in Age Associated Morbidity. In conjunction with a neutral sphingomyelinase and the subsequent synthesis of diacylglycerol thence promoting activation of a PKC-Zeta, T cell receptor translocation and activation are obtained. Prior RAG mediated recombination events in CNS-resident lymphocytes, associated microglia and neurons can lead to recombination event epigenomi...
Authentic Biochemistry Neuroimmune and Epigenetic Interactions in Aging III. DJGPhD 21.4.21
April 21, 2021 21:56 - 29 minutes - 27.4 MBSingle-nucleotide polymorphisms and copy-number variations (CNV’s) result from genome wide chromosomal abnormalities including large deletions and duplications where the the immune system appears to play a major role in the accumulation of chromosomal variation. Aging in humans may be linked to immune-mediated epigenomic epistasis as facilitated by Recombination Activating Gene proteins RAG1 & RAG2 that are canonically linked to V(D)J recombination of the T cell receptor and the B cell i...
The immune system is both genetically programmed and environmentally adaptable to epigenetic states. Lecture II DJGPhD 19 April 2021
April 20, 2021 01:54 - 29 minutes - 27.2 MBNeuroinflammatory mediators play a critical role in the pathophysiology of brain ischemia, exerting either deleterious effects on the progression of tissue damage or beneficial roles during recovery and repair. These processes occur throughout life and involve the immune system, biochemical pathways, cellular turnover and alteration of gene expression. Signal transduction cascades allow for genome editing to epigenomic mediation of metabolic event ontologies. Aging is a process whereby th...
The Immuno-Epigenome Contribution to Aging Foundation Lecture I. DJGPhD 18.4.21
April 18, 2021 22:54 - 29 minutes - 27.2 MBAn Introduction to the theory of immunoepigenetic tailoring of human instanence. Dr. Guerra Authentic Biochemistry Podcast Studios --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
T lymphocyte caveolae and the efficiency of effector cell differentiation. DJGPhD 4.4.21 Authentic Biochemistry
April 04, 2021 22:24 - 28 minutes - 26.3 MBJ Immunol August 1, 2017, 199 (3) 874-884 Ann Rev Cell Dev Biol 2018. 34:111-136 Blood. 2016 Apr 14;127(15):1930-9 J BiolChem. 2017 Aug 25; 292(34): 14292–14307 --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Involving Instanence. A Philosophical Episode on Authentic Biochemistry. DJGPhD 31 March 2021
March 31, 2021 21:04 - 23 minutes - 21.1 MBWhat is involved to make a sound decision? Biochemistry is a scientific discipline; one that examines and interprets the foundational structures and functions of living systems. As with any judgment, as there are reasons for believing, there are also reasons for disbelieving, and the freely willed decision to choose which, should involve the discovery of truths to use as exemplars for seeking coherence and foundation to the premises that necessarily and sufficiently support the better argu...
Caveolae and Alzheimer's Disease. The Micro-vasculature Paradigm: Endothelial Cell Corruption in the CNS Authentic Biochemistry . Dr Guerra. 26 March 2021
March 27, 2021 00:34 - 29 minutes - 27.4 MBAnnual Review of Cell and Developmental Biology Vol. 34:111-136 Journal of Neuroscience 23 October 2019, 39 (43) 8576-8583 Archivum Immunologiae et Therapiae Experimentalis 2013 Apr;61(2):119-25. --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Lipoprotein synthesis, turnover and trafficking in concert with caveolae-mediated lipid transport. Dr Dan Guerra Authentic Biochemnistry. 25.3.21.
March 25, 2021 23:37 - 29 minutes - 27.4 MBIn this foundational lipid absorption lecture, Dr. Guerra address the roles of apo- lipoproteins, triacylglycerol and cholesteryl esters with membrane associated caveolae transport in adipocytes, endothelia and skeletal muscle. An Authentic Biochemistry Podcast Production. 25 March 2021. --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Condensed Bioenergetics : Dr Guerra Drives the Electron Transport Chain via Standard Oxidation Reduction Potentials. 21.3.21
March 22, 2021 01:10 - 29 minutes - 27.3 MBElectron transport and the anapleurotics of intermediary metabolism. Dr. Daniel J. Guerra Authentic Biochemistry Studios 21.3.21 --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Landing on epithelial-mesenchymal transition and T lymphocyte selection to explore Senescence-Associated Cancer-like Stem Cells. DJGPhD 20.3.21
March 21, 2021 01:28 - 29 minutes - 27.2 MB•Impaired immunological responsiveness is common in the elderly •The immunological competence of an individual is determined by the presence of mature lymphocytes formed in primary lymphoid organs, and secondary lymphoid tissues such as the liver, lung and GI tract. •Immunological equilibrium requires steady lymphocyte output, and controlled expansion •Thymic and lymph-node stromal microenvironments thus represent key elements in the development of the adaptive immune system. Consequently...
ADP- Glucokinase is essential for T lymphocyte activation and pro-inflammatory cytokine synthesis and secretion. DJGPhD. 19.3.21
March 19, 2021 23:43 - 29 minutes - 27.3 MB•Cytokines regulate the intensity and duration of the immune response, and they can act as growth factors or stimulants or even inhibitors of T cell amplification and differentiation. •Cytokines are glycoproteins that are synthesized through a dolichol PP mediated pathway in the ER and are thus dependent upon prenyl lipid metabolism Endoplasmic reticulum localized ADPGK serves glycoprotein synthesis and also functions to control glycolytic vs. aerobic bioenergetic intermediary metabolic a...
The control of C flux in the liver, pancreas and CNS are coordinated: Potential Axis for Immuno Senescence X Intermediary Metabolic Regulation.Authentic Biochemistry . 15 March 2021. DJG PhD.
March 16, 2021 00:17 - 29 minutes - 27 MB1. Glucokinase- GK is in the neuron 2.Control over glycolysis via GK mediates neural transmission 3. Neuronal GK further functions on the appetite/satiety axis thus partially regulating the HPA axis 4. Aging and immune associated dysfunction of these glycolytic systems are associated with neurodegeneration and the loss of neuronal action potential mediated learning, memory, and mood. Therefore, metabolic control over the severity of chronological age and the immunosenescence of the CNS...
The pyridoxal phosphate contribution to amino acid metabolism and neurotransmitter turnover Dr Guerra Authentic Biochemistry 10 March 2021
March 11, 2021 00:05 - 29 minutes - 27.3 MBIf γ-aminobutyric acid (GABA) concentration drops significantly in the synaptic cleft, excitotoxicity can obtain, sometimes presenting with epileptic convulsions.This can be reversed by decreasing the concentration of L-glutamate via control over the activity of glutamate dehydrogenase, glutamine synthetase and glutamate transporters or by the inhibition of γ-aminobutyric acid aminotransferase (GABA-AT), which catalyzes the conversion of GABA to the excitatory neurotransmitter l-glutamic ...
Reactive Oxygen and the gamma-glutamyl cycle for GSH redox biochemistry and the prolegomena of neurodegeneration. DJGPhD 09March21'
March 09, 2021 20:54 - 29 minutes - 26.6 MBA basic lecture on reactive oxygen synthesis and reduction coupled with glutathione, folic acid and amino acid metabolism. This lecture is foundational for subsequent synthesis with neural transmission, microglial activation and exicitotoxicity leading to neurodegeneration in the aging human CNS. Please subscribe, rate and review, and contribute to Authentic Biochemistry! [email protected] Published 9 March 2021 by Dr Daniel J. Guerra. --- Support this podcast: https://podcasters.spot...
Deacylation regulation of amino acid catabolism as a source of multiple bioenergetic and discrete excitotoxicity phenomena. DJGPhD . 03March2021
March 04, 2021 01:27 - 29 minutes - 26.9 MBCPS-1 activity is regulated by liver enriched transcription factors as well as Sirtuin-mediated de-acylation. Glutaminase breaks down glutamine into glutamate and ammonia. Glutamate also yields additional NH4+ via the enzyme glutamate dehydrogenase. From here, ammonia is initially incorporated into hepatocyte mitochondria and ultimately results in the formation of urea. Urea subsequently leaves the hepatocyte cytoplasm and is ultimately excreted in urine. Glutaminase-1 (GLS1) is a mitocho...
Amino Acid Catabolism via Sirtuins Modulation and the Redirecting of Hepatic and Intestinal Intermediary Metabolism. Dr Guerra Authentic Biochemistry. 02 March 2021
March 02, 2021 20:04 - 29 minutes - 27.4 MBGlutaminase breaks down glutamine into glutamate and ammonia. Glutamate also yields additional urea via the enzyme glutamate dehydrogenase. From here, ammonia is initially incorporated into hepatocyte mitochondria and ultimately results in the formation of urea. Urea subsequently leaves the hepatocyte cytoplasm and is ultimately excreted in urine. Sirtuin mediated control over glutamate dehydrogenase and carbamoyl-P synthetase via discrete and potentiating ADP ribosylation and deacylation ...
Sirtuins obtain a significant role in mitochondrial metabolism linked to caloric restriction and cancer. Dr Guerra. Authentic Biochemistry.26.02.21
February 26, 2021 23:45 - 29 minutes - 27.5 MBSIRT5 causes deglutarylation and functional activation of glutamate dehydrogenase 1 which is essential to cellular glutaminolysis.Indeed, SIRT5 supports the anaplerotic transamination entry of glutamine and other amino acids as alpho ketoglutarate into the TCA cycle in malignant phenotypes of colorectal cancer via activation of the glutamate dehydrogenase1. Sirt5 is known to regulate the activity of the urea cycle enzyme, carbamoyl phosphate synthase 1 (CPS1). SIRT5 mediated de-glutarylat...
Thermodynamics and regulation involving lipoic acid biochemistry of the classical 2-oxo acid dehydrogenase complexes. Dr Guerra 23.02.2021
February 23, 2021 21:41 - 29 minutes - 27.4 MBThe Pyruvate dehydrogenase hydrated E1-E2 interface is enthalpy driven, while the dehydrated E3-peripheral subunit binding domain complex is driven by entropy obtaining a favourable delta G= delta H-T delta S= -33.4kj mol where the domain interfacial hydration obtains surface thermal complementarity and contributes finally to an aggregate strength of multiple affinities of individual non-covalent binding interactions via enthalpy-driven catalysis. Lipoamidase activity of mitochondria...
Physical Biochemistry of Free Energy Transfer for Membrane Transport of Molecules and Ions.Dr Dan Guerra 19 Feb 2021
February 20, 2021 01:58 - 29 minutes - 27.4 MB•Free energy change(chemical potential difference) for transporting 1 mole of a substance from region where its concentration is C1 (e.g., Cout) to region where its concentration is C2 (e.g., Cin): ∆G= RTln(C2/C1) (favorable with ∆G< 0 if C2< C1) •Transport of ionsacross membrane (must consider electrical potential in addition to concentration difference): ∆G= RTln(C2/C1) + ZF ∆Y (Z=charge of ion, F=Faraday’s constant, ∆Y=membrane electrical potential in volts) •Coupled transport (act...
Membrane Proteins 1 Mini Lecture Dr Guerra 19 Feb 2021
February 20, 2021 00:41 - 19 minutes - 18.1 MBMini Lecture 1 on Membrane Physiological Biochemistry. The amino acid sequence of membrane associated polypeptides obtains secondary structure, glycosylation, acylation and hydropathy which confer functionality (in part) by targeting specific membrane domains. --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Application of the Principle of Sufficient Reason to Biochemical Thermodynamics. Dr Dan Guerra 13 February 2021
February 14, 2021 00:51 - 29 minutes - 27.4 MBFor Valentine's Day Eve. A bouquet assortment of biochemical thermodynamic principles and metaphysical event ontologies. To finish my thoughts: Is life necessary? How to answer this question? One way is to ask if there is sufficient reason for its existence. Outside of spiritual or theological discussion, the answer is compellingly obscure especially since we find no authentic examples except on our planet. If life is contingent, where does it come from, how does it come about and wh...
Cell Cycle kinase inhibition may improve healthy cardiac aging while promoting global tumorigenesis. Authentic Biochemistry 10Feb2021 by Dr Guerra.
February 11, 2021 00:10 - 29 minutes - 27.3 MB*Oncogenesis eventuates genetic mutation to epigenetic gene expression mechanisms with molecular signatures either inappropriately proscribed, incorrectly prescribed upon writing as dangerous and deleterious, or erasure; leaving a corrupted chromatin result. *Tumor cells obtain proliferative autonomy, autophagous –self-maintenance in growth and signaling, neovascularization for nutrient and oxygen supply, and resistance to anti-proliferative and apoptotic stimuli *In resting cells, th...
Adipokine Update Lecture 2. Authentic Biochemistry Podcast. Dr. Dan Guerra Published 06 Feb 2021
February 07, 2021 02:44 - 29 minutes - 27.1 MBIntra abdominal adiposity (IAA) linked obesity may be the most significant contributory factor to high mortality human disease after aging. IAA presents with systemic and chronic inflammation and enlarged White Adipose Tissue (WAT) adipocytes that have been infiltrated with lymphocytes and macrophages that secrete pro-inflammatory cytokines, chemokines, growth factors and inappropriate levels of pro-inflammatory adipokines. Aging combined with chronic obesity not only promotes cardiovas...
Adipokine Mini Review I Daniel J. Guerra, PhD. 03 Feb 2021 Authentic Biochemistry Podcast Publications.
February 04, 2021 01:10 - 29 minutes - 27 MB*Adipose tissue is the endocrine organ that secretes “adipokines” *Nothing new to the Authentic Biochemistry crew; adipokines have been very well discussed here and their function as mediators of the feeding/appetitive/satiety response through the arcuate nucleus of the hypothalamus (POMCvNPY neurons) was most recently addressed. *Adipokines mediate the BMI, heart rate, serum glucose &fatty acid/TAG, and pro- inflammatory cytokine production;they are released by adipocytes (e.g. leptin and...
Neuropeptide Y and the POMC neuronal antagonism in feeding behaviour maps onto fear conditioning. Dr. Dan Guerra 28 January 2021
January 28, 2021 21:29 - 29 minutes - 27.2 MBProceedings of The Nutrition Society.2012 71(4):521-33 Autophagy. 2009 May; 5(4): 558–560 Molecular and Cellular Endocrinology Volume 472, 5 September 2018, Pages 40-49 Int J Mol Sci. 2020 Nov; 21(21): 8220. --- Support this podcast: https://podcasters.spotify.com/pod/show/dr-daniel-j-guerra/support
Neuropeptide Y and the fear conditioning response: are aging and obesity prodromal to anxiety and cognitive decline? Daniel J. Guerra PhD. Published 25 January 2021
January 25, 2021 22:31 - 29 minutes - 27.3 MBFear-conditioning learning is a low-threshold enduring psychiatric process that prepares a defense against dangerous phenomena and reduces the need to iteratively relearn the signal. It is a pattern recognition response that can be modulated by experience and the severity of the stress signal and it is a response that deteriorates in the elderly and in certain neuropsycoses and anxiety disorders throughout life. Fear conditioning must be fluid to readjust according to reverse learning...
Corticotropin Releasing Factor and the Monoamine Fear Response in Aging. Sequence II. The reverse learning scenario as cognitive redistribution of neural transmission valency. DJGPhD.21.01.2021.
January 21, 2021 22:21 - 29 minutes - 27 MBHomeostatic chronic low stress results in low to moderate levels of CRF in the LC in association with enhanced Extra-Dimensional Shifting and optimal executive decision making. However, acute or chronic severe stress is linked to high levels of CRF in the LC and this may contribute to a shift from optimal executive function necessary for goal-directed behavior toward an iterative decision response. In healthy environments, this variable tonicity is a readout for adaptation when goal-...
The Corticotropin Releasing Factor and the Somatosensory Catecholamine Circuit in ExtraDimensional (attentional) Shifting. Dr Guerra 20 Jan 2021.AB.
January 20, 2021 21:30 - 28 minutes - 26.5 MBAdaptation to stress-such as the fear response obtains dopaminergic input to striatum and prefrontal cortex and is thought to signal unexpected events and facilitate a shift in attention to promote new learning within the contralateral primary somatosensory cortex (SI) which has been associated with the agentic categories of both the quality and quantity of thought event ontology. This process works in conjunction with the bilateral secondary somatosensory cortex (SII) process involved in...
Aging-associated SASP vs. MiDAS is a contrarion trade-off that may lead to neurodegeneration and autoinflammation vs tumorigenesis and heart disease respectively. Dr. Dan Guerra.17 Jan 2021. AB
January 17, 2021 21:43 - 27 minutes - 25.5 MBThe anti-inflammatory IL-10 pathway in the CNS can lead to Glioblastoma which is a disease presented 3-4 fold higher in people ≥65 years old, and with a mortality rate for the same age group some 7 fold higher. Activation of the IL-10 Receptor bound ligand induces the JAK1 signal transducer and activator of transcription 3 (STAT3) pathway in APCs. This results in the subsequent translocation of STAT3 homodimers into the nucleus. This STAT3 homodimer binds to STAT-binding elements which promo...
An Immunological Distinction between Senescence linked Secretory Phenotypes: SASP vs. Midas. Dr. Dan Guerra. Authentic Biochemistry 16 Jan 2021
January 17, 2021 02:08 - 29 minutes - 27.4 MBNon-uniform ageing is a major associative risk factor for cancer and degenerative diseases and mitochondrial dysfunction is linked to cellular senescence in association with cell cycle arrest, telomerase decline and nucleic acid/lipid/protein oxidation. Indeed, mitochondrial dysfunction comprises a distinct type of cellular senescence; mitochondrial SIRT3 or SIRT5, can induce Mitochondrial Dysfunction Associated-Senescence (MiDAS) Senescence is a chronological and pathophysiological sequenc...
The stress-induced Neural Network tracks Kantian Epistemology through characteristic action potential and neuromodulator/neurotransmitter phenomenology. Dr Guerra 13 JAN 2021.
January 14, 2021 00:52 - 28 minutes - 26.5 MBLC axonal projections to the mPFC modulate a diversity of cognitive processes, including working memory, sustained attention, and flexible attention and under moderate rates of LC activity and NE release, high-affinity postsvnaptic α2 adrenergic receptors in the PFC are preferentially engaged and promote working memory However, with elevated LC firing and activation of lower affinity α1 adrenergic receptors, working memory is impaired while agentic focused attention becomes prominent so it ...
Fear and Aging associated with neuronal conditioning and synaptogenesis via corticotropin releasing hormone. Dr. Dan Guerra; Authentic Biochemistry 13 JAN 2021
January 13, 2021 19:38 - 29 minutes - 26.9 MB•Intensive and chronic or excessive stress diminishes intellectual performance and general cognition: this is a form of negative reinforcement and can be related to learning disability and anxiety about future events. Indeed, •Individual responses and the magnitude of stressor as well as its association with self identity and goals will influence endurance, resilience, and self-empowerment vs. dissatisfaction and defeat. •Stresses first become recognized in the developing fetus since the f...
Fear associated Corticotropin Releasing Factor and the Immune response are linked to CNS neutral Sphingomyelinase I. Dr Dan Guerra 09 January 2021
January 10, 2021 01:27 - 30 minutes - 27.5 MB1. Corticotropin releasing factor (CRF) or hormone (CRH) is one of several neurohormones synthesized by specific hypothalamic nuclei in the brain and released into the portal system which bathes the anterior pituitary 2. CRF has marked CNS effects by acting at higher centers in the brain: cortical regions where there is a widespread distribution of CRF neurons. 3. Major role of CRF is to prepare the organism for an appropriate response to various stressors such as physical trauma, insults ...
Glycolysis is inhibited in T cells when Glucose 6 P non-competitively inhibits hexokinase after GLUT1 uptake of serum glucose:Setting the stage for lipogenesis.
January 07, 2021 02:20 - 29 minutes - 26.8 MBNaïve T Cell metabolic regulationinvolves allosteric and kinetic mechanisms for maximum capacity to switch from glucose uptake to fatty acylCoA utilization for bioenergetic demand. Multiple mechanisms of this coordinated regulation involve the fatty acyl CoA concentration modulation by fine tuning cyctosolic fatty acid synthesis from glucose or lactate or amino acids vs. mitochondrial beta oxidation to drive the ATP concentration to levels necessary to prepare for cell proliferation and ev...
The Porosomal complex and complex lipid interconversions organizing the transport and secretion of cytokines after T cell Receptor Activation by APC's
January 06, 2021 03:20 - 29 minutes - 26.8 MBThere is a requirement of cholesterol and ceramides for the maintenance of neuronal and T cell porosome integrity, accompanying an interaction of phosphatidic acid (PA) and polyphosphoinositide (PI) lipids with syntaxin-1A where these lipids are necessary for neurotransmitter or cytokine secretion in association with calcium channels modulated by lipid domain formation upon sphingomelinase and fatty acyltransferase activities. Complex lipid composition and three dimensional sequence t...
Inhibitory PFK1 palmitoylation is facillitated by Acid Sphingomyelinase. Daniel J. Guerra, PhD. Authentic Biochemistry Podcast 02 January 2021
January 03, 2021 02:36 - 29 minutes - 27.1 MBCellular long chain acyl-CoA concentrations are controlled by multiple networked and coherent metabolic pathways ( including mitochondrial β-oxidation versus Golgi-ER-PL complex lipid synthesis versus glyceropholipid and sphingolipid hydrolysis), which functionally associate resident acyl-CoA in cellular bioenergetic and signaling pathways. Thus, the regulation of PFK-1 by its direct interaction with acyl-CoA generates downstream interconnected alterations in metabolic flux through glycol...
T lymphocyte maturation and memory cell accumulation are differentially mediated by sphingomyelinase and protein palmitoylation. Dr Dan Guerra. 01 Jan 2021
January 01, 2021 20:22 - 29 minutes - 27.2 MBNaive T lymphocytes, as opposed to memory T cells(which are more common in aging) carry out homolactate fermentitive glycolysis. Memory cells are operating under complete aerobic metabolism involving mitochondrial bioenergetics including the TCA cycle but also fatty acid oxidation as a source for reducing equivelents to drive the electrontransport chain and oxidative phosphorylation. The regulation of these alternative pathways involves allosteric modification of key enymes and traffick...
Tumor cells and T lymphocytes carry forward initially similar and subsequently distinct bioenergetic regulation through AMPK. Dr Guerra Authentic Biochemistry 30 Dec. 2020
December 30, 2020 20:25 - 29 minutes - 27.2 MBEarly stage TCR activated T cells with rampant lactic acid fermentation, fail to maintain adequate ATP levels , especially when glucose is limiting.This decrease in ATP relative to ADP+AMP results in the activation of AMPK, which inhibits mTOR activity and all subsequent anabolism especially assocaited for T cell proliferation. Indeed, induced Loss of AMPKα1, will sythetically restore mTOR signaling and T cell cytokine production, but not proliferation. Although loss of AMPK can restore ...
Tumor cells and T lymphocytes cary forward initially similar and subsequently distinct bioenergetic regulation through AMPK. Dr Guerra Authentic Biochemistry 30 Dec. 2020
December 30, 2020 20:25 - 29 minutes - 27.5 MBEarly stage TCR activated T cells with rampant lactic acid fermentation, fail to maintain adequate ATP levels , especially when glucose is limiting.This decrease in ATP relative to ADP+AMP results in the activation of AMPK, which inhibits mTOR activity all all sunsequent anabolism especially assocaited for T cell proliferation. Indeed, induced Loss of AMPKα1, will sythetically restore mTOR signaling and T cell cytokine production, but not proliferation. Although loss of AMPK can restore ...
T cell metabolic Dialectics : deriving bioenergetic supremecy from glycolysis through fatty acid and lipid synthesis to ultimate fatty acid oxidation and ATP generation. Dr Daniel J. Guerra
December 30, 2020 01:35 - 29 minutes - 27.2 MBAuthentic Biochemistry Podcast 29 December 2020 Daniel J. Guerra Ph.D. •All scientific inquiry should start with dialectical method that uses the current knowledge base to generate various specific Theses and then follow each with the counter-argument by employing the Square of Opposition thus producing Anti-theses and then, making the third movement, which allows for a Synthesis that has the flavor of rejection, acceptance or indifference. *Downstream of co-stimulation and PI3K-AKT, the ...
On the Concept of Neuro-Correlation: A Theoretical Essay. Dr. Daniel J. Guerra . Authentic Biochemistry. 25 December 2020
December 26, 2020 01:57 - 22 minutes - 20.5 MBHuman neuroscientific research seeks to uncover patterns of commonality and uniformity to help define mechanisms which reliably result in the experience of thinking and interacting with the world. This scientific mindset is in opposition to real life experience where human bonding is unique to a specific relationship. This may be best understood as a compatibilist approach, where neuroscience may affirm the very real experience of an individual when he makes a judgment or chooses to imagine ...
T Cell signal transduction involves kinase and transcription factor mediated expression of bioenergetic ensemble gene products. Dr DJ Guerra 23 December 2020
December 23, 2020 23:29 - 29 minutes - 27.1 MBT cell agency initiates upon the stimulation of the TCR via presentation of cognate peptide-MHC complexes in associative ligand-mediated membrane co-receptor CD28 with co-stimulatory molecules presented on the surface of the APC: this event is called T cell licensure and essentially quits quiescence. The TCR signals through the ERK/MAPK pathways and calcium flux; where as CD28 signaling activates the PI3K-AKT-mTOR axis, and both pathways synergistically engage the NF-κB organon of pleitropi...
From Glucose to Triacylglycerol in the developing T lymphocyte. Dr Daniel J. Guerra. Authentic Biochemistry 21 December 2020
December 23, 2020 22:39 - 29 minutes - 27 MBIn this episode Dr Guerra provides a concise description of T lymphocytic fatty acid synthesis from glucose via cytosolic glycolysis, mitochondrialpyruvate dehydrogenase, pyruvate carboxylase, and citrate synthase and a return to the cytosol for ATP citrate lyase and the homodimeric polyprotein complex FAS to generate palmitoyl-ACP. This ability of antigen-MHC Antigen Presenting Cell TCR - activated T lymphocytes to prepare for massive cytokine and chemokine expression requires a bioche...
The Misguided Linkage of Potentially Ablating Aging -Associated Metabolic Syndrome by tracking NAD+ serum levels. Dr. Daniel Guerra. 17 December 2020.
December 17, 2020 21:48 - 29 minutes - 26.9 MBMetabolic syndrome (MetSyn)is a dyslipidaemic chronic and aging linked sedentary life-style constellation of disease presentations including metabolic disorganization, prodromal Type 2 diabetes and a prominent connection to hypertension-most prominently associated with over-eating and obesity. MetSyn is often prolegomena to middle-age onset CVD, cancer and neurodegeneration. High caloric density soluble carbohydrate nutrition, lack of fasting and the species-specific metabolic inability t...
Intermediary metabolism plays an essential role in immune cell activation that may lead to tissue degeneration, lipid accumulation and oncogenesis in the aging human. Dr. Guerra. 14 December 2020.
December 15, 2020 00:11 - 29 minutes - 27.2 MBBoth the innate and acquired immune systems become senescent during chronological aging. While T cell activation may become disenfranchised from its co-stimulatory control apparatus during aging, the potential for hyper or hypo-immune responses becomes more common. At the same time, CNS microglia and peripheral resident and circulating macrophages are activated following the stimulation of various pattern recognition or phagocytic receptors. In the CNS, this activation state is subsequent...