PubReading
346 episodes - English - Latest episode: 10 months ago -Researchers would love if they can expand time to fit in that publication, article, or review of their topic of interest; if that were true, however, there is no end to bottomless scrolling and missing the important details. PubReading would read out the abstract, results, and discussions to allow hassle-free information and a chance to create connections with like-minded individuals.
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Episodes
PubReading [45] - Built-In Active Microneedle Patch with Enhanced Autonomous Drug Delivery - M.A. Lopez-Ramirez, J. Wang et al.
October 25, 2021 20:00 - 40 minutes - 74 MBThe use of microneedles has facilitated the painless localized delivery of drugs across the skin. However, their efficacy has been limited by slow diffusion of molecules and often requires external triggers. Herein, an autonomous and degradable, active microneedle delivery platform is introduced, employing magnesium microparticles loaded within the microneedle patch, as the built-in engine for deeper and faster intradermal payload delivery. The magnesium particles react with the interstitial...
PubReading [44] - I-motif DNA structures are formed in the nuclei of human cells - M. Zeraati, D. Christ et al.
October 23, 2021 20:00 - 30 minutes - 56.7 MBHuman genome function is underpinned by the primary storage of genetic information in canonical B-form DNA, with a second layer of DNA structure providing regulatory control. I-motif structures are thought to form in cytosine-rich regions of the genome and to have regulatory functions; however, in vivo evidence for the existence of such structures has so far remained elusive. Here we report the generation and characterization of an antibody fragment (iMab) that recognizes i-motif structures ...
PubReading [43] - Click and Cut: a click chemistry approach to developing oxidative DNA damaging agents - N. McStay, A. Kellett et al.
October 22, 2021 20:00 - 59 minutes - 109 MBMetallodrugs provide important first-line treatment against various forms of human cancer. To overcome chemotherapeutic resistance and widen treatment possibilities, new agents with improved or alternative modes of action are highly sought after. Here, we present a click chemistry strategy for developing DNA damaging metallodrugs. The approach in- volves the development of a series of polyamine ligands where three primary, secondary or tertiary alkyne-amines were selected and ‘clicked’ using...
PubReading [42] - Circulating tumoral DNA: Preanalytical validation and quality control in a diagnostic laboratory - S. Nikolaev, T. Nouspikel et al.
October 20, 2021 23:00 - 27 minutes - 50.7 MBWe present the results of our technical validation process in establishing the analysis of circulating tumor DNA (ctDNA) as a diagnostic tool. Like most cells in our body, tumor cells shed DNA in the blood flow. Analysis of ctDNA mutational content can provide invaluable information on the genetic makeup of a tumor, and assist oncologists in deciding on therapy, or in following residual disease. However, low absolute amounts of circulating DNA and low tumor fraction constitute formidable ana...
PubReading [41] - Apoptosis: A Target for Anticancer Therapy - C. M. Pfeffer and A. T. K. Singh
October 20, 2021 20:00 - 31 minutes - 57.7 MBApoptosis, the cell’s natural mechanism for death, is a promising target for anticancer therapy. Both the intrinsic and extrinsic pathways use caspases to carry out apoptosis through the cleavage of hundreds of proteins. In cancer, the apoptotic pathway is typically inhibited through a wide variety of means including overexpression of antiapoptotic proteins and under-expression of proapoptotic proteins. Many of these changes cause intrinsic resistance to the most common anticancer therapy, c...
PubReading [40] - Phosphoproteome profiling uncovers a key role for CDKs in TNF signaling - M. Tanzer, M. Mann et al
October 19, 2021 20:00 - 53 minutes - 97.8 MBTumor necrosis factor (TNF) is one of the few cytokines successfully targeted by therapies against inflammatory diseases. However, blocking this well studied and pleiotropic ligand can cause dramatic side-effects. Here, we reason that a systems-level proteomic analysis of TNF signaling could dissect its diverse functions and offer a base for developing more targeted therapies. Therefore, we combine phosphoproteomics time course experiments with sub-cellular localization and kinase inhibitor ...
PubReading [39] - Crystal structure of a membrane-bound metalloenzyme that catalyses the biological oxidation of methane - R. L. Lieberman and A. C. Rosenzweig
October 18, 2021 22:14 - 36 minutes - 67.1 MBParticulate methane monooxygenase (pMMO) is an integral membrane metalloenzyme that catalyses the conversion of methane to methanol. Knowledge of how pMMO performs this extremely challenging chemistry may have an impact on the use of methane as an alternative energy source by facilitating the development of new synthetic catalysts. We have determined the structure of pMMO from the methanotroph Methylococcus capsulatus (Bath) to a resolution of 2.8A ̊. The enzyme is a trimer with an a3b3g3 po...
PubReading [38] - PeptiCHIP: A Microfluidic Platform for Tumor Antigen Landscape Identification - S. Feola, V. Cerullo et al.
October 16, 2021 20:00 - 1 hour - 118 MBIdentification of HLA class I ligands from the tumor surface (ligandome or immunopeptidome) is essential for designing T-cell mediated cancer therapeutic approaches. However, the sensitivity of the process for isolating MHC-I restricted tumor-specific peptides has been the major limiting factor for reliable tumor antigen characterization, making clear the need for technical improvement. Here, we describe our work from the fabrication and development of a microfluidic-based chip (PeptiCHIP) a...
PubReading [37] - High resolution single particle cryo-electron microscopy using beam-image shift - A. Cheng, B. Carragher et al.
October 15, 2021 20:00 - 23 minutes - 42.3 MBAutomated data acquisition is used widely for single-particle reconstruction of three-dimensional (3D) volumes of biological complexes preserved in vitreous ice and imaged in a transmission electron microscope. Automation has become integral to this method because of the very large number of particle images required in order to overcome the typically low signal-to-noise ratio of these images. For optimal efficiency, automated data acquisition software packages typically employ some beam-imag...
PubReading [36] - APE1: A skilled nucleic acid surgeon - A. Whitaker and B. Freudenthal
October 14, 2021 20:00 - 39 minutes - 71.7 MBBefore a deleterious DNA lesion can be replaced with its undamaged counterpart, the lesion must first be removed from the genome. This process of removing and replacing DNA lesions is accomplished by the careful coordination of several protein factors during DNA repair. One such factor is the multifunctional enzyme human apurinic/apyrimidinic endonuclease 1 (APE1), known best for its DNA backbone cleavage activity at AP sites during base excision repair (BER). APE1 preforms AP site incision ...
PubReading [35] - Oligonucleotide conjugated multi-functional adeno-associated viruses - D. Katrekar, P. Mali et al.
October 13, 2021 20:00 - 26 minutes - 49.1 MBRecombinant adeno-associated viruses (AAVs) are among the most commonly used vehicles for in vivo gene delivery. However, their tropism is limited, and additionally their efficacy can be negatively affected by prevalence of neutralizing antibodies in sera. Methodologies to systematically engineer AAV capsid properties would thus be of great relevance. In this regard, we develop here multi-functional AAVs by engineering precision tethering of oligonucleotides onto the AAV surface, and thereby...
PubReading [34] - Replication protein A binds RNA and promotes R-Loop formation - O. Mazina, A. V. Mazin et al.
October 12, 2021 20:00 - 33 minutes - 60.4 MBReplication protein A (RPA), a major eukaryotic ssDNA- binding protein, is essential for all metabolic processes that involve ssDNA, including DNA replication, repair, and damage signaling. To perform its functions, RPA binds ssDNA tightly. In contrast, it was presumed that RPA binds RNA weakly. However, recent data suggest that RPA may play a role in RNA metabolism. RPA stimulates RNA-templated DNA repair in vitro and associates in vivo with R-loops, the three-stranded structures consisting...
PubReading [33] - Oxidative DNA Cleavage with Clip-Phenanthroline Triplex-Forming Oligonucleotide Hybrids - A. Panattoni, M. Hocek et al.
October 11, 2021 20:00 - 39 minutes - 72.9 MBA systematic study of several new types of hybrids of Cu-chelated clamped phenanthroline artificial metallonuclease (AMN) with triplex-forming oligonucleotides (TFO) for sequence-specific cleavage of double-stranded DNA (dsDNA) is reported. The synthesis of these AMN–TFO hybrids is based on application of the alkyne–azide cycloaddition click reaction as the key step. The AMN was attached through different linkers at either the 5’- or 3’-ends or in the middle of the TFO stretch. The diverse ...
PubReading [32] - Enhancing Protein Crystallization under a Magnetic Field - S. Young Ryu, H. Kyu Song et al.
October 08, 2021 22:17 - 22 minutes - 41.7 MBHigh-quality crystals are essential to ensure high-resolution structural information. Protein crystals are controlled by many factors, such as pH, temperature, and the ion concentration of crystalline solutions. We previously reported the development of a device dedicated to protein crystallization. In the current study, we have further modified and improved our device. Exposure to external magnetic field leads to alignment of the crystal toward a preferred direction depending on the magneti...
PubReading [31] - TLR8 Is a Sensor of RNase T2 Degradation Products - W. Greulic, T. Carell, V. Hornung et al.
October 07, 2021 22:37 - 55 minutes - 102 MBTLR8 is among the highest-expressed pattern-recognition receptors in the human myeloid compartment, yet its mode of action is poorly understood. TLR8 engages two distinct ligand binding sites to sense RNA degradation products, although it remains unclear how these ligands are formed in cellulo in the context of complex RNA molecule sensing. Here, we identified the lysosomal endoribonuclease RNase T2 as a non-redundant upstream component of TLR8- dependent RNA recognition. RNase T2 activity i...
PubReading [30] - Cone-shaped HIV-1 capsids are transported through intact nuclear pores - V. Zila, M. Beck et al.
October 06, 2021 20:00 - 47 minutes - 86.4 MBHuman immunodeficiency virus (HIV-1) remains a major health threat. Viral capsid uncoating and nuclear import of the viral genome are critical for productive infection. The size of the HIV-1 capsid is generally believed to exceed the diameter of the nuclear pore complex (NPC), indicating that capsid uncoating has to occur prior to nuclear import. Here, we combined correlative light and electron microscopy with subtomogram averaging to capture the structural status of reverse transcription-co...
PubReading [29] - Using antibodies to control DNA-templated chemical reactions - L. B. Pellejero, F. Ricci, T. Brown Jr et al.
October 05, 2021 20:00 - 34 minutes - 63.8 MBDNA-templated synthesis takes advantage of the programmability of DNA-DNA interactions to accelerate chemical reactions under diluted conditions upon sequence-specific hybridization. While this strategy has proven advantageous for a variety of applications, including sensing and drug discovery, it has been so far limited to the use of nucleic acids as templating elements. Here, we report the rational design of DNA templated synthesis controlled by specific IgG antibodies. Our approach is bas...
PubReading [28] - DNA G‐quadruplexes in the human genome: detection, functions and therapeutic potential - R. Hänsel-Hertsch, M. Di Antonio and S. Balasubramanian
October 04, 2021 20:00 - 32 minutes - 59.7 MBSingle-stranded guanine-rich DNA sequences can fold into four-stranded DNA structures called G-quadruplexes (G4s) that arise from the self-stacking of two or more guanine quartets. There has been considerable recent progress in the detection and mapping of G4 structures in the human genome and in biologically relevant contexts. These advancements, many of which align with predictions made previously in computational studies, provide important new insights into the functions of G4 structures ...
PubReading [27] - Rapid Generation of Long Noncoding RNA Knockout Mice Using CRISPR/Cas9 Technology - N. R. Hansmeier, J.W. Kornfeld et al.
October 01, 2021 22:29 - 24 minutes - 45.1 MBIn recent years, long noncoding RNAs (lncRNAs) have emerged as multifaceted regulators of gene expression, controlling key developmental and disease pathogenesis processes. However, due to the paucity of lncRNA loss-of-function mouse models, key questions regarding the involvement of lncRNAs in organism homeostasis and (patho)-physiology remain difficult to address experimentally in vivo. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 platform provides a powerful...
PubReading [26] - Tools and methods for circular dichroism spectroscopy of proteins: a tutorial review - A. J. Miles, Robert W. Janes and B. A. Wallace
September 29, 2021 21:00 - 1 hour - 121 MBCircular dichroism (CD) spectroscopy is a widely-used method in biochemistry, structural biology and pharmaceutical chemistry. More than 24000 papers published in the past decade have included CD characterisations of proteins; many of those studies have also included other complementary chemical, biophysical, and computational chemistry methods. This tutorial review describes the background to the technique of CD spectroscopy and good practice methods for high quality data collection. It spe...
PubReading [25] - Brown adipose tissue monocytes support tissue expansion - A. Gallerand, Laurent Yvan-Charvet1 & Stoyan Ivanov et al.
September 28, 2021 21:00 - 47 minutes - 87.4 MBMonocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments...
PubReading [25] - Brown adipose tissue monocytes support tissue expansion - A. Gallerand, L. Yvan-Charvet1 & S. Ivanov et al.
September 28, 2021 21:00 - 47 minutes - 87.4 MBMonocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments...
PubReading [24] - Root of the Tree: The Significance, Evolution, and Origins of the Ribosome - J. C. Bowman, L. D. Williams et al.
September 27, 2021 21:00 - 1 hour - 181 MBThe ribosome is an ancient molecular fossil that provides a telescope to the origins of life. Made from RNA and protein, the ribosome translates mRNA to coded protein in all living systems. Universality, economy, centrality and antiquity are ingrained in translation. The translation machinery dominates the set of genes that are shared as orthologues across the tree of life. The lineage of the translation system defines the universal tree of life. The function of a ribosome is to build riboso...
PubReading [23] - Deformylation of 5-Formylcytidine in Different Cell Types - E. Korytiakovà and T. Carell
September 09, 2021 21:41 - 26 minutes - 47.7 MBEpigenetic programming of cells requires methylation of deoxycytidines (dC) to 5-methyl-dC (mdC) followed by oxidation to 5-hydroxymethyl-dC (hmdC), 5-formyl-dC (fdC), and 5-carboxy-dC (cadC). Subsequent transformation of fdC and cadC back to dC by various pathways establishes a chemical intra-genetic control circle. One of the discussed pathways involves the Tdg-independent deformylation of fdC directly to dC. Here we report the synthesis of a fluorinated fdC feeding probe (F-fdC) to study ...
PubReading [22] - Shining Light on CRISPR Gene Editing - L. Taemaitree and T. Brown
September 07, 2021 21:09 - 9 minutes - 17.4 MBAdvanced spatiotemporal control of CRISPR-Cas9 activity is demonstrated through the use of chemically modified photoactivatable guide RNA. - doi.org/10.1021/acscentsci.0c00350 - 2020
PubReading [21] - DNA structure from A to B - R.Dickerson and HL. Ng
September 06, 2021 22:13 - 14 minutes - 26.6 MBP. Shing Ho and his colleagues at Oregon State and Berkeley publish in this issue of PNAS an interesting study (1) of helical structure in the DNA hexamer GGCGCC, finding that various states that appear to be logical intermediates between A-DNA and B-DNA can be induced by methylation or bromination of cytosine or by crystal packing. Their results bear on three issues that have been argued over in the past: (i) the differences between A-DNA and B- DNA and transitions between them, (ii) the in...
PubReading [20] - Anatomy, Back, Cauda Equina - E. Berg, J. Ashurst.
September 06, 2021 22:02 - 11 minutes - 21.6 MBIn 1595, French anatomist Andre du Laurens first described the structure of a rope-like tail of fibers at the caudal end of the spinal cord. This bundle of numerous axons was termed the cauda equina, from the Latin translation meaning “horse’s tail,” and it contains nerves which innervate both sensory and motor targets within lumbar, sacral, and coccygeal spinal cord levels. Epidemiologic assessments regard lesions to the cauda equina as uncommon, with a prevalence of 1 to 3 per 100,000 subj...
PubReading [19] - Hospitalization Length after Myocardial Infarction: Risk-Assessment-Based Time of Hospital Discharge vs. Real Life Practice - Michał Wegiel and Tomasz Rakowski
September 02, 2021 22:13 - 21 minutes - 39.7 MBAccording to guidelines, it is safe for low-risk patients with myocardial infarction (MI) to be discharged within 72 h of hospitalization. However, results coming from registries show that the hospital stay is often much longer in a real-life situation. Data on the length of the hospital stay (LOS) of MI patients in Polish centers are lacking. We enrolled 212 consecutive patients with acute MI. Low-risk patients were defined according to PAMI II criteria: age 45%, no persistent ventricular a...
PubReading [18] - Controlling and enhancing CRISPR systems - H. Shivram, B. Cress, G. Knott, J. Doudna
September 01, 2021 22:53 - 48 minutes - 88.1 MBMany bacterial and archaeal organisms use CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR associated) systems to defend themselves from mobile genetic elements. These CRISPR-Cas systems are classified into six types based on their composition and mechanism. CRISPR-Cas enzymes are widely used for genome editing and offer immense therapeutic opportunity to treat genetic diseases. To realize their full potential, it is important to control the timing, duration, effi...
PubReading [17] - Antisense, RNAi, and gene silencing strategies for therapy: Mission possible or impossible? - E. Rayburn and R. Zhang
August 31, 2021 22:24 - 36 minutes - 66.5 MBAntisense oligonucleotides can regulate gene expression in living cells. As such, they regulate cell function and division, and can modulate cellular responses to internal and external stresses and stimuli. Although encouraging results from preclinical and clinical studies have been obtained and significant progress has been made in developing these agents as drugs, they are not yet recognized as effective therapeutics. Several major hurdles remain to be overcome, including problems with eff...
PubReading [16] - Measuring cancer evolution from the genome - T. Graham and A. Sottoriva -
August 30, 2021 18:51 - 41 minutes - 75.9 MBThe temporal dynamics of cancer evolution remain elusive, because it is impractical to longitudinally observe cancers unperturbed by treatment. Consequently, our knowledge of how cancers grow largely derives from inferences made from a single point in time – the endpoint in the cancer’s evolution, when it is removed from the body and studied in the laboratory. Fortuitously however, the cancer genome, by virtue of ongoing mutations that uniquely mark clonal lineages within the tumour, provide...
PubReading [15] - Engineering antibody therapeutics - M. Chiu and G. Gilliland
August 24, 2021 00:30 - 43 minutes - 79.3 MBThe successful introduction of antibody-based protein therapeutics into the arsenal of treatments for patients has within a few decades fostered intense innovation in the production and engineering of antibodies. Reviewed here are the methods currently used to produce antibodies along with how our knowledge of the structural and functional characterization of immunoglobulins has resulted in the engineering of antibodies to produce protein therapeutics with unique properties, both biological ...
PubReading [14] - Acute abdominal pain in patients with Crohn’s disease: What urgent imaging tests should be done? - P. García, A. López-Jurado, A. Bártulos
August 20, 2021 23:42 - 11 minutes - 20.9 MBCrohn’s disease is an autoimmune disease that predominantly affects the gastrointestinal tract. Crohn’s disease is diagnosed at a young age and runs a chronic course with acute flare-ups. When patients with Crohn’s disease present with flare-ups at the emergency department, they are usually managed in a way similar to patients with acute abdomen; there is no consensus about the most appropriate imaging work-up for patients with flare-ups of Crohn’s disease. Thus, we decided to review the lit...
PubReading [13] - Herpesvirus capsid assembly and DNA packaging - J. Heming, J. Conway and F. Homa
August 19, 2021 23:30 - 51 minutes - 94.8 MBHerpes simplex virus type I (HSV-1) is the causative agent of several pathologies ranging in severity from the common cold sore to life-threatening encephalitic infection. During productive lytic infection, over 80 viral proteins are expressed in a highly regulated manner, resulting in the replication of viral genomes and assembly of progeny virions. The virion of all herpesviruses consists of an external membrane envelope, a proteinaceous layer called the tegument, and an icosahedral capsid...
PubReading [12] - Ruthenium Complexes as Anticancer Agents: A Brief History and Perspectives - S. Lee, C. Kim, T.G. Nam
August 18, 2021 21:53 - 59 minutes - 108 MBPlatinum (Pt)-based anticancer drugs such as cisplatin have been used to treat various cancers. However, they have some limitations including poor selectivity and toxicity towards normal cells and increasing chemoresistance. Therefore, there is a need for novel metallo-anticancers, which has not been met for decades. Since the initial introduction of ruthenium (Ru) polypyridyl complex, a number of attempts at structural evolution have been conducted to improve efficacy. Among them, half-sand...
PubReading [11] - Outcomes of critically ill patients with acute kidney injury in COVID-19 infection: an observational study - Rodrigo Bezerra et al.
August 17, 2021 20:16 - 32 minutes - 59.2 MBBackground: Early reports indicate that AKI is common during COVID-19 infection. Different mortality rates of AKI due to SARS-CoV-2 have been reported, based on the degree of organic dysfunction and varying from public to private hospitals. However, there is a lack of data about AKI among critically ill patients with COVID-19. Methods: We conducted a multicenter cohort study of 424 critically ill adults with severe acute respiratory syndrome (SARS) and AKI, both associated with SARS-CoV-2, ...
PubReading [9] - Principles of ubiquitin and SUMO modifications in DNA repair - S. Bergink, S. Jentsch
August 13, 2021 23:31 - 37 minutes - 68.3 MBWith the discovery in the late 1980s that the DNA-repair gene RAD6 encodes a ubiquitin-conjugating enzyme, it became clear that protein modification by ubiquitin conjugation has a much broader significance than had previously been assumed. Now, two decades later, ubiquitin and its cousin SUMO are implicated in a range of human diseases, including breast cancer and Fanconi anaemia, giving fresh momentum to studies focused on the relationships between ubiquitin, SUMO and DNA-repair pathways. -...
PubReading [10] - Recommendations on pre-hospital and early hospital management of acute heart failure - short version - Alexandre Mebazaa et al.
August 12, 2021 23:16 - 17 minutes - 32.2 MBDespite several critical steps forward in the management of chronic heart failure (CHF), the area of acute heart failure (AHF) has remained relatively stagnant. As stated in the updated ESC HF guidelines, clinicians responsible for managing patients with AHF must frequently make treatment decisions without adequate evidence, usually on the basis of expert opinion consensus.2 Specifically, the treatment of acute HF remains largely opinion-based with little good evidence to guide therapy. Acut...
PubReading [8] - A Glimpse of Structural Biology through X-Ray Crystallography - Yigong Shi
August 06, 2021 17:55 - 1 hour - 153 MBSince determination of the myoglobin structure in 1957, X-ray crystallography, as the anchoring tool of structural biology, has played an instrumental role in deciphering the secrets of life. Knowledge gained through X-ray crystallography has fundamentally advanced our views on cellular processes and greatly facilitated development of modern medicine. In this brief narrative, I describe my personal understanding of the evolution of structural biology through X-ray crystallography—using as ex...
PubReading [7] - Modified nucleic acids: replication, evolution, and next-generation therapeutics - K. Duffy, S. Arangundy-Franklin and P. Holliger
August 05, 2021 23:04 - 43 minutes - 79.8 MBModified nucleic acids, also called xeno nucleic acids (XNAs), offer a variety of advantages for biotechnological applications and address some of the limitations of first-generation nucleic acid therapeutics. Indeed, several therapeutics based on modified nucleic acids have recently been approved and many more are under clinical evaluation. XNAs can provide increased biostability and furthermore are now increasingly amenable to in vitro evolution, accelerating lead discovery. Here, we revie...
PubReading [6] Will CRISPR‐Cas9 Have Cards to Play Against Cancer? An Update on its Applications - P Daisy, K Shreyas, T. S. Anitha - Molecular Biotechnology
August 03, 2021 00:08 - 1 hour - 122 MBGenome editing employs targeted nucleases as powerful tools to precisely alter the genome of target cells and regulate functional genes. Various strategies have been risen so far as the molecular scissors-mediated genome editing that includes zinc finger nuclease, transcription activator-like effector nucleases, and clustered regularly interspaced short palindromic repeats—CRISPR-related protein 9. These tools allow researchers to understand the basics of manipulating the genome, create anim...
PubReading [5] G-Quadruplexes at Telomeres: Friend or Foe? - Tracy Bryan - Molecules
July 28, 2021 22:05 - 51 minutes - 93.5 MBTelomeres are DNA-protein complexes that cap and protect the ends of linear chromosomes. In almost all species, telomeric DNA has a G/C strand bias, and the short tandem repeats of the G-rich strand have the capacity to form into secondary structures in vitro, such as four-stranded G-quadruplexes. This has long prompted speculation that G-quadruplexes play a positive role in telomere biology, resulting in selection for G-rich tandem telomere repeats during evolution. There is some evidence t...
PubReading [4] Triplex-forming oligonucleotides: a third strand for DNA nanotechnology - Arun Chandrasekaran, David Rusling - Nucleic Acids Research
July 27, 2021 00:26 - 1 hour - 166 MBDNA self-assembly has proved to be a useful bottom- up strategy for the construction of user-defined nanoscale objects, lattices and devices. The design of these structures has largely relied on exploiting simple base-pairing rules and the formation of double-helical domains as secondary structural elements. However, other helical forms involving specific non-canonical base-base interactions have introduced a novel paradigm into the process of engineering with DNA. The most notable of these ...
PubReading [3] DNA Structure: A-, B- and Z-DNA Helix Families - David W Ussery - Encyclopedia Of Life Science
July 22, 2021 23:30 - 26 minutes - 48.9 MBThere are three major families of DNA helices: A-DNA, B-DNA and Z-DNA. The helical structure of DNA is variable and depends on the sequence as well as the environment. David W Ussery
PubReading [2] New Approaches Towards Recognition of Nucleic Acid Triple Helices - Dev P. Arya - Acc Chem Res
July 20, 2021 23:32 - 43 minutes - 80 MBWe show that groove recognition of nucleic acid triple helices can be achieved with aminosugars. Among these aminosugars, neomycin is the most effective aminoglycoside (groove binder) for stabilizing a DNA triple helix. It stabilizes both the T·A·T triplex and mixed-base DNA triplexes better than known DNA minor groove binders (which usually destabilize the triplex) and polyamines. Neomycin selectively stabilizes the triplex (T·A·T and mixed base) without any effect on the DNA duplex. The se...
PubReading [1] A structure for Deoxyribose Nucleic Acid - J. D. Watson, F. H. C. Crick - Nature
July 17, 2021 12:16 - 7 minutes - 14.5 MBMolecular Structure of Nucleic Acids - A structure for Deoxyribose Nucleic Acid - We wish to suggest astructure for the salt of deoxyribose nucleic acid (D.N.A.). This structure has novel features which are of considerable biological interest. J. D. Watson, F. H. C Crick.