Separation and Purification Techniques
MCAT Basics (from MedSchoolCoach)
English - May 09, 2024 08:00 - 1 hour - 58.7 MB - ★★★★★ - 222 ratingsMedicine Health & Fitness Education Courses becomingaphysician gettingintomedicalschool improvingmcatscore mcat mcatscore medicalschool medschool Homepage Download Apple Podcasts Google Podcasts Overcast Castro Pocket Casts RSS feed
This episode of MCAT Basics covers several separation and purification techniques you may see on the MCAT. We’ll cover these techniques in three distinct categories: small molecules, proteins, and cells.
Within the small molecule category, we’ll discuss extraction, crystallization and recrystallization, and chromatography (TLC and HPLC).
For proteins, we cover SDS-page, precipitation, soelectric focusing, and chromatography variations. Lastly, we’ll explain, within the world of cells, centrifugation, flow cytometry, and microfluidics.
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[00:00] MCAT Tutorig from MedSchoolCoach
[00:34] Welcome to MCAT Basics
[01:07] Topics covered in this episode
[02:24] Techniques for small molecules - extraction, crystallization and recrystallization, and chromatography (TLC and HPLC)
[28:24] Techniques for proteins - SDS-page, precipitation, isoelectric focusing, and chromatography variations
[54:47] Techniques for cells - centrifugation, flow cytometry, and microfluidics.