MSACL
21 episodes - English - Latest episode: 6 months ago - ★★★★★ - 1 ratingWhat's happening in the clinical lab community including publications in JMSACL and interviews with the Early Career Network.
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Episodes
The Regulatory Landscape of Laboratory Developed Tests (LDT): A Laboratorian Perspective
November 14, 2023 17:00 - 56 minutes - 51.7 MBThe FDA released a proposed LDT rule and stated it will “end its enforcement discretion” of LDTs. Previously, the FDA explored other avenues for regulating LDTs. We will discuss questions relevant to the draft rule and implications to laboratory medicine, including, but not limited to: - What are possible implications of FDA regulation of LDTs? - Is the FDA the appropriate mechanism for this LDT oversight? - What about modernization of CLIA? - What would be the financial cost? - What would be...
MSACL 2023 : A Chat with Jennifer Van Eyk on her Distinguished Contribution Award Lecture
April 05, 2023 16:30 - 12 minutes - 11.1 MBDustin Bunch chats with Jennifer Van Eyk on the fine points of her Distinguished Contribution Award lecture from the previous evening. Recorded onsite at MSACL 2023 Monterey in the Exhibit Hall.
MSACL 2023 : A Chat with Tim Collier on his Michael S Bereman Award Lecture
April 05, 2023 16:00 - 11 minutes - 10.6 MBDustin Bunch chats with Tim Collier on some of the nuances of his Michael S Bereman Award for Innovative Clinical Proteomics lecture from the previous afternoon. Recorded onsite at MSACL 2023 Monterey in the Exhibit Hall.
The MetaProteomics Initiative : The Cornerstone of Comprehensive Molecular Analysis of the Microbiome Ecosystem
March 16, 2023 17:00 - 41 minutes - 38.4 MBThrough connecting genomic and metabolic information, metaproteomics is an essential approach for understanding how microbiomes function in space and time. The international metaproteomics community is delighted to announce the launch of the Metaproteomics Initiative (www.metaproteomics.org), the goal of which is to promote dissemination of metaproteomics fundamentals, advancements, and applications through collaborative networking in microbiome research.
Paper Spray Mass Spectrometry in Clinical Application
December 15, 2022 00:00 - 34 minutes - 31.6 MBAn interview with Lindsey Kirkpatrick, DO, PhD and Nicholas Manicke, PhD discussing the technology behind, and development of, Paper Spray technology, as well as its place in the clinical lab. Read the companion article in JMSACL, Development and validation of a paper spray mass spectrometry method for the rapid quantitation of remdesivir and its active metabolite, GS-441524, in human plasma (https://doi.org/10.1016/j.jmsacl.2022.06.001).
The triple quadrupole: Innovation, serendipity and persistence
November 02, 2022 21:00 - 42 minutes - 39.1 MBAn interview with Richard Yost, co-developer of the Triple Quadrupole Mass Spectrometer. How it was developed and why. Read the companion article in JMSACL at https://www.sciencedirect.com/science/article/pii/S2667145X22000141
JMSACL Journal Club : Supervised Machine Learning for Mass Spectrometry Data Analysis: Experts' Opinion
March 01, 2022 18:00 - 58 minutes - 54 MBWe will be talking with Thomas Durant and Edward Lee about recent advances in machine learning (ML) for mass spectrometry (MS) data analysis. The primary focus will be the alignment of these two fields (ML and MS) and how this offers a promising synergy that can be used to optimize workflows, improve result quality, and enhance our understanding of high-dimensional datasets, as well as their inherent relationship with disease biology. We will also dig deeper to understand a basic overview of...
Fireside Chat with Christopher Rose on Single Cell Proteomics
September 29, 2021 17:00 - 1 hour - 42.4 MBChristopher Rose shares his thoughts on single cell proteomics.
JMSACL Journal Club : Understanding X-Chromosome Deletion Disorder using metabolomics and lipidomics
August 20, 2021 05:26 - 1 hour - 53.4 MBX linked disorders are considerably rare and research analysing human samples is under-represented. Research undertaken in this study used neural progenitor cells from an afflicted patient to begin exploring this rare disorder. While most experimental work focuses on the neurodevelopmental impacts of X-chromosome associated diseases, this work demonstrates that they should also be considered metabolic disorders owing to their perturbations on metabolite and lipid biochemistry. This work aims...
Novel Techniques using Silicon (in silico) and Gold to Improve Imaging Results
April 29, 2021 16:30 - 1 hour - 56.6 MBUntargeted small molecule machine learning for MSI Cameron Shedlock (until 14:00) Mass spectrometry imaging returns large dimensionally complex datasets. Traditional analysis in imaging utilizes a mindset focused on target molecule identification that parses data with a narrow view of molecular exploration. These data analysis methods are specifically focused on exploring differences between a traditional organic acid matrix when compared to using nanoparticles for MSI, which results in ve...
JMSACL Journal Club with Benjamin Owusu
April 23, 2021 17:03 - 59 minutes - 54.3 MBA presentation and discussion with Dr. Owusu on his recently published paper in JMSACL entitled Development and validation of a novel LC-MS/MS assay for C-peptide in human serum.
Electrochemical Strategies in ESI-MS for Lipid Analysis
April 22, 2021 07:00 - 1 hour - 67.4 MBLipids play a vital role in maintaining cellular functions. Altered lipid metabolism is currently considered a hallmark of many diseases, which highlights the importance of the characterization of lipid composition in understanding, diagnosing, and treating pathologies. Discrimination of isomeric species is challenging in lipidomics. In this talk, I will introduce the microdroplet electrochemical methods capable of resolving different types of isomers commonly encountered in lipid samples us...
Reference data-driven metabolomics and application to nutrition research
April 20, 2021 17:00 - 1 hour - 71.8 MBSurveying the food-associated compounds detected in clinical samples, we can differentiate patients with specific diet types, such as predominantly animal protein- versus plant-based diet. In addition, we can identify specific foods associated with clinical outcomes in disease cohorts. With its broad applications, we envision this approach becoming invaluable in nutrition research as well as many other fields once additional reference datasets become available.
Empirically tracking the exposure of human plasma & serum samples to thawed conditions
April 06, 2021 17:00 - 1 hour - 70.2 MBThis presentation will described the concept, methodology, validation and performance characteristics of a 10-µL, dilute-and-shoot, intact-protein mass spectrometric assay of albumin proteoforms called “Delta-S-Cys-Albumin” that serves as an endogenous marker of P/S exposure to thawed conditions based on the inexorable ex vivo S-cysteinylation (oxidizability) of albumin. The multi-reaction chemical mechanism that drives changes in albumin S-cysteinylation is known and the rate law for it was...
Getting going with MS (4) : Josh attempts method validation
March 09, 2021 18:00 - 1 hour - 93.8 MBThis session will outline the studies conducted and planned in order to complete validation of a mass spectrometry method in a CAP-accredited, clinical laboratory. While accuracy and precision and reportable range will be addressed, particular attention will be paid to the additional validation studies that should be conducted (matrix effect, injector stability, interferences, etc).
Getting going with MS (3) : Josh analyzes peaks
February 11, 2021 16:00 - 1 hour - 89.3 MBUnlike conventional immunoassays which provide simple (and often incorrect!) results, mass spectrometers provide a plethora of data for every peak- qualifier ions, internal standard areas, retention times, peak shapes, etc. This data, often termed metadata, can help clinical laboratories evaluate their peaks to ensure that they are reporting accurate results. Unfortunately, the process of evaluating this metadata can be a doubting challenge. This session will discover our efforts to move ahe...
Getting going with MS (2) : Josh learns chromatography
January 07, 2021 18:00 - 1 hour - 89.1 MBWhat do we want? Good chromatography! How do we get it? We don’t know! Columns, maybe? Good chromatography is essential to any LC-MS/MS method. Setting it up is often one of the earlier steps in developing a new method. While it can be an iterative process, setting up good chromatographic conditions at the start can set you up for success later on. This podcast will discuss our laboratories efforts to setup chromatographic conditions for the analysis of ethyl-glucuronide and buprenorphine (...
Getting going with mass spectrometry : Josh learns chromatography
January 07, 2021 18:00 - 1 hour - 89.1 MBWhat do we want? Good chromatography! How do we get it? We don’t know! Columns, maybe? Good chromatography is essential to any LC-MS/MS method. Setting it up is often one of the earlier steps in developing a new method. While it can be an iterative process, setting up good chromatographic conditions at the start can set you up for success later on. This podcast will discuss our laboratories efforts to setup chromatographic conditions for the analysis of ethyl-glucuronide and buprenorphine (...
Vitamin D and Immune Function
December 08, 2020 18:00 - 1 hour - 82 MBProf. Hewison’s group is at the forefront of research linking vitamin D and the immune system, with implications for a wide range of clinical disorders including infectious, inflammatory and autoimmune disease. Current studies are focused on the analysis of vitamin D-insensitivity in T lymphocytes from the inflamed joints of patients with rheumatoid arthritis. This may provide an explanation for the limited success of vitamin D supplementation in some clinical trials. Other projects have exp...
Getting going with MS (1) : Josh installs a mass spectrometer
October 22, 2020 17:00 - 50 minutes - 45.9 MBThis session will serve as an introduction to a 4 part series in which Dr. Hayden will invite attendees to witness in real time his journey bringing mass spec testing to a clinical lab. During these interactive sessions, attendees will be encouraged to help troubleshoot, and offer advice as desired. Subsequent sessions anticipated include: 1. Getting going with mass spectrometry: Josh learns chromatography 2. Getting going with mass spectrometry: Josh tries to do sample preparation 3. Gett...
Getting going with mass spectrometry: Josh installs a mass spectrometer
October 22, 2020 17:00 - 50 minutes - 45.9 MBThis session will serve as an introduction to a 4 part series in which Dr. Hayden will invite attendees to witness in real time his journey bringing mass spec testing to a clinical lab. During these interactive sessions, attendees will be encouraged to help troubleshoot, and offer advice as desired. Subsequent sessions anticipated include: 1. Getting going with mass spectrometry: Josh learns chromatography 2. Getting going with mass spectrometry: Josh tries to do sample preparation 3. Gett...