PubReading [324] - DNA sequencing with chain-terminating inhibitors - F. Sanger, S.Nickel & A. Coulson
PubReading
English - May 09, 2023 11:01 - 17 minutes - 16.1 MBLife Sciences Science Health & Fitness Medicine Homepage Download Apple Podcasts Google Podcasts Overcast Castro Pocket Casts RSS feed
A new method for determining nucleotide sequences in DNA is described. It is similar to the "plus and minus" method but makes use of the 2',3 '- dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase. The technique has been applied to the DNA of bacteriophage phiX174 and is more rapid and more accurate than either the plus or the minus method.doi: 10.1073/pnas.74.12.5463. - 1977