Mentors at Your Benchside
133 episodes - English - Latest episode: almost 2 years ago -A good mentor can be the difference between success and failure. But what to do if you don't have help on hand? Mentors At Your Benchside provides curated help and advice from experienced researchers on various topics, from lab skills and techniques to career progression.
Each short episode is bursting with easy-to-access help and advice that can improve your results and help you get the most out of your time in the lab.
https://bitesizebio.com/
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Episodes
How (and Why) To Manipulate Plasmid Copy Number
August 16, 2022 11:00 - 8 minutes - 7.48 MB#17 — Why do some plasmids give a better protein yield than others? It may have a lot to do with the plasmid copy number. In this episode, we talk about what plasmid copy number means, why it is important and how you can manipulate it to get the most out of your experiments. To learn more about plasmid copy number, read the full article on our site. [1] Resources: 1. https://bitesizebio.com/22824/how-to-manipulate-plasmid-copy-number/
Top 10 Tips for Viva Success
August 11, 2022 11:00 - 6 minutes - 5.9 MB#16 — Is your viva on the horizon? Many people think viva exams are going to be painful. But what if you could make your viva go smoothly and maybe even enjoy it? Listen to our top 10 viva tips to get advice from a viva survivor. To learn more about surviving your viva, read the full article on our site. [1] Resources: 1. https://bitesizebio.com/10109/top-10-tips-for-viva-success/
A Brief History of CRISPR-Cas9 Genome-Editing Tools
August 09, 2022 11:00 - 7 minutes - 6.7 MB#15 — In the last few years, CRISPR-Cas9 has become a popular genome editing tool, but have you ever wondered where it came from? In this episode, we explore the history of CRISPR-Cas9, from the first CRISPR discovery in 1987 to current applications in targeted genome and epigenome editing. Read the full article to learn more about the history of CRISPR-Cas9 and to see a timeline of discoveries. [1] Resources: 1. https://bitesizebio.com/47927/history-crispr/
8 Top tips for Improving Accuracy and Precision
August 04, 2022 11:00 - 9 minutes - 8.89 MB#14 — Do you know how accurate and precise your measurements are? Imprecise and inaccurate measurements can have a dramatic impact on your results. Listen to get 8 top tips for making your measurements more accurate and precise. To learn more about accuracy and precision, read the full article on our site. [1] Resources: 1. https://bitesizebio.com/55470/accuracy-and-precision/
Choosing a Fluorescent Protein
August 02, 2022 10:00 - 14 minutes - 13.3 MB#13 — There are now hundreds of different tailored fluorescent proteins to choose from that can improve your experiments in the lab. What are the key considerations when choosing a fluorescent protein for your experiment? Should you consider using a fancy new fluorescent protein? Where can you find the best resources to help you out? Find out the answers to these and more in this episode of Mentors At Your Benchside. Please read the full article, which includes our super helpful table of ...
DNA Precipitation: Ethanol vs. Isopropanol
July 28, 2022 10:00 - 6 minutes - 5.91 MB#12 — As a follow-up to our episode about ethanol precipitation of DNA and RNA, [1] this episode explains the differences between DNA precipitation in ethanol and isopropanol, helping you to figure out which method is the best choice for your experiment. Read the full article for handy protocol tips, the differences between using ethanol and isopropanol, and when to use each method. [2] Resources: 1. https://bitesizebio.com/2007/12/04/the-basics-how-ethanol-precipitation-of-dna-and-rna-work...
How Fluorescent Molecules Work
July 26, 2022 10:00 - 6 minutes - 5.6 MB#11 — Fluorescence is undoubtedly one of the most important and useful tools in a biologist’s toolbox. But do you actually know how fluorescence works? In this episode, discover what the three steps of fluorescence are, and how fluorescence can be used in flow cytometry. Read the full article for a breakdown of the key points of fluorescence. [1] Resources: 1. https://bitesizebio.com/32973/fluorescent-molecules/
Which Type of Ethanol Should You
July 21, 2022 10:00 - 4 minutes - 4.38 MB#10 — As you probably know, there are different types of ethanol found in biology labs. It's a versatile solvent used in dozens of experiments and procedures, including disinfection, DNA precipitation, and tissue dehydration. But which type of ethanol should you use for your application? What's the difference between the types of ethanol in your lab? And how do you handle it appropriately? In this episode, we answer these questions, providing you with the information you need to keep your...
What the HEK? A Beginner’s Guide
July 19, 2022 10:00 - 6 minutes - 6.21 MB#9 — One of the most commonly used cell lines in molecular biology labs is the Human Embryonic Kidney HEK293 cell line. Listen to this episode to find out all about the history of HEK293 cells and how to work with them. Read the full article for more details about working with HEK293 cells. [1] Resources: 1. https://bitesizebio.com/45489/guide-to-hek293-cells/
How SDS-PAGE Works
July 14, 2022 11:00 - 7 minutes - 7.23 MB#8 — You probably have or will use SDS-PAGE at some point to separate proteins, but do you really understand how this technique works? Knowing how SDS-PAGE works means you can tweak and troubleshoot your technique as well as impress your supervisor and lab mates. In this episode, we take you through how SDS-PAGE works, including what SDS does, why you need to use a reducing agent like DTT or beta-mercaptoethanol, and the critical importance of the stacking gel. Read the full How SDS-PAGE Wo...
How a Jellyfish Changed Biology: the Discovery and Developme
July 12, 2022 11:00 - 5 minutes - 4.99 MB#7 — In this episode, we cover how jellyfish were able to have a huge impact on biology through the discovery and development of GFP. Using GFP is now ubiquitous in pretty much all fields in biology, and we take you through how three Nobel Laureates developed this valuable research tool. [1] Many of the applications for GFP are microscopy-based, and we discuss how you can utilize GFP for translational and transcriptional fusions, FRAP, FLIP and FRET experiments in the lab. [2,3] Read the f...
Ethanol Precipitation of DNA and RNA and How it Works
July 07, 2022 11:00 - 5 minutes - 4.98 MB#6 — Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acid preparations in an aqueous solution. In this episode, we'll bring you up to speed on how ethanol precipitation works, including the importance of solubility, the roles of salt, ethanol, and temperature, plus a few helpful tips on the side. Read the full article to learn more about the ins and outs of ethanol precipitation and other DNA clean-up approaches. https://bitesizebio.com/253/the-ba...
20 Telling Signs You're a Scientist
July 05, 2022 11:00 - 4 minutes - 4.27 MB#5 — Have you ever chilled beer on dry ice from the lab? Does your wardrobe consist mainly of free T-shirts from companies at conferences? (https://bit.ly/conference-who-where-what) Have you ever wondered what the distilled water in the lab tastes like? (https://bit.ly/water-what-is-it-good-for) If any of these questions apply to you, then tune in to this episode, as we cover more funny and telling signs that you're a true scientist. Read the full article to discover the difference between...
What is PCR? The Beginner’s Guide
June 30, 2022 11:00 - 10 minutes - 9.61 MB#4 — PCR is a useful lab technique used to copy, sequence or quantify DNA. If you're new to PCR, this episode is for you. We cover the five things you need to get started with PCR and explain how to run a polymerase chain reaction. Read the full article to learn more about setting up a PCR. [1] Learn more about Kary Mullis and the invention of PCR, and about the first thermocycler "Mr Cycle". [2–4] For practical tips, see our article on the top tips for primer design. [5] Resources: 1. Wh...
A Short History of Cryo-Electron Microscopy
June 28, 2022 11:15 - 9 minutes - 8.36 MB#3 — In this episode of Mentors At Your Benchside, listen to a short history of cryo-electron microscopy—the Nobel Prize-winning technique revolutionizing molecular and structural biology. The journey takes us from the inception of cryo-EM- obscure, inferior, and derided- to the present-day competition for access to the incredibly powerful Krios microscopes. It's all about the power of an image. And speaking of images, be sure to read A Short History of Cryo-Electron Microscopy for a gr...
17 Ways to Stop Pipetting Errors From Ruining Experiments
June 28, 2022 11:00 - 9 minutes - 9.1 MB#2 — Micropipettes are the bread and butter instrument of a lab, helping you to accurately dispense minute amounts of liquid. But these tools are only trustworthy if they are well maintained and handled appropriately. Listen to the latest episode of Mentors At Your Benchside to uncover top tips for precise pipetting. Want to know more about correct pipetting technique? Access the original article on 17 Ways to Avoid Pipetting Errors, download the Gilson Guide to Pipetting, and read the Natur...
Is It Worth Doing A PhD After A Masters?
June 28, 2022 10:45 - 6 minutes - 5.79 MB#1 — Is it worth doing a PhD? This is a question that probably plagues every research student at some point in their career. In this episode, we explore 5 important questions you should consider before embarking on a PhD. Read the full article for more advice before making the difficult decision to pursue a PhD after getting your Master’s degree. [1] If you are looking for further advice, make sure you check out our article with pointers for PhD students. [2] A good PhD supervisor is worth...
The World of Macrophages: How to Harness Them for Research
July 23, 2019 17:15 - 59 minutes - 54.3 MBAlthough macrophages were first described by Elie Metchnikoff in 1882, plenty of mysteries are still associated with the cell type. Indeed, while macrophages were once considered simply "garbage trucks" of the immune system due to their phagocytic property, their substantial and multifaceted contribution to immunological responses and homeostasis is becoming more apparent. Macrophages can produce a wide range of cytokines and chemokines to influence the immune response toward healing or infla...
Basics of FLIM and Applications to FRET Biosensors
June 16, 2019 19:18 - 50 minutes - 34.4 MBFunctional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to reveal the underlying cellular mechanisms. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artefacts and sensitive to molecular environment such has pH changes, ion concentrations, and more. Förster Resonance Energy Tran...
Ep 58 — Basics of FLIM and Applications to FRET Biosensors
June 16, 2019 19:18 - 50 minutes - 34.4 MBFunctional imaging is a rapidly growing field key to driving new understanding in biology. Insights into the function and interaction of molecules are the key to reveal the underlying cellular mechanisms. In this context, fluorescence lifetime imaging (FLIM) is a powerful tool, providing valuable information beyond spectral imaging. FLIM is immune to concentration artefacts and sensitive to molecular environment such has pH changes, ion concentrations, and more. Förster Resonance Energy Tran...
Adding Multiplex Factor to Confocal Imaging with ZEISS LSM 9
May 14, 2019 16:00 - 48 minutes - 33.1 MBIn this tutorial on confocal imaging, you will learn how you can: - Capture weaker signals—and still get sound, reproducible data. - Reach faster volumetric imaging without sacrificing resolution - Increase data throughput for your imaging needs Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellul...
Ep 57—Adding Multiplex Factor to Confocal Imaging with ZEISS LSM
May 14, 2019 05:00 - 48 minutes - 33.1 MBIn this tutorial on confocal imaging, you will learn how you can: - Capture weaker signals—and still get sound, reproducible data. - Reach faster volumetric imaging without sacrificing resolution - Increase data throughput for your imaging needs Learn about the new Multiplex mode for parallel pixel acquisition with the ZEISS LSM 9 family and Airyscan 2. You can now acquire up to 8 superresolution image lines with high signal-to-noise rate in a single sweep. Capture dynamic processes, cellul...
Cryo-electron Tomography for Cell Biology
April 11, 2019 18:00 - 58 minutes - 40.4 MBIn this webinar, you will learn: - The complete workflow for in situ cryo-electron tomography - How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) - How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of proteaso...
Ep 56 — Cryo-electron Tomography for Cell Biology
April 11, 2019 05:00 - 58 minutes - 40.4 MBIn this webinar, you will learn: - The complete workflow for in situ cryo-electron tomography - How subtomogram averaging within the cell yields native-state structures of macromolecular complexes (e.g., the asymmetric and dilated nuclear pore of algae) - How mapping these structures back into the native cellular environment reveals new molecular interactions that are only accessible by this technique (e.g., the binding of cargo to COPI-coated Golgi membranes and the tethering of proteaso...
Cryo-Electron Microscopy and the Complexity of Cancer
March 28, 2019 17:00 - 58 minutes - 40.1 MBIn this webinar, you will learn: - How structural biology can change the face of your research - The biological challenges of cancer research - Advantages of cryo-EM for your research Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure. In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of in...
Ep 55 — Cryo-Electron Microscopy and the Complexity of Cancer
March 28, 2019 05:00 - 58 minutes - 40.1 MBIn this webinar, you will learn: - How structural biology can change the face of your research - The biological challenges of cancer research - Advantages of cryo-EM for your research Covering both biology and methodology, this webinar will explain how single-particle cryo-electron microscopy enables us to gain insight into cancer development through the detailed analysis of molecular structure. In single-particle cryo-EM, hundreds of thousands of images formed by electron scattering of in...
Fast superresolution microscopy – with Lattice SIM
March 05, 2019 16:00 - 56 minutes - 38.5 MBYour life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and many different labels. In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffraction limit of conventional microscopy to image your samples with superresolution. Learn how to examine the fastest processes in living samples – in ...
Ep 54 — Fast superresolution microscopy – with Lattice SIM
March 05, 2019 05:00 - 56 minutes - 38.5 MBYour life sciences research often requires you to measure, quantify and understand the finest details and sub-cellular structures of your sample. You may be working with tissue, bacteria, organoids, neurons, living or fixed -cells and many different labels. In this webinar, we will explain how Elyra 7 with Lattice SIM takes you beyond the diffraction limit of conventional microscopy to image your samples with superresolution. Learn how to examine the fastest processes in living samples – in ...
Array Tomography for SEM 3D Reconstruction
November 27, 2018 17:45 - 1 hour - 41.8 MBArray tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of your array tomography: - Fast and precise trimming of the sample block-face - Adhesion of a single section to create ribbons - Automated serial sectioning with the ARTOS 3D ultramicrotome - Acquisition and proce...
Ep 53 — Array Tomography for SEM 3D Reconstruction
November 27, 2018 05:00 - 1 hour - 41.8 MBArray tomography (AT) is a 3D image reconstruction technique for high resolution, quantitative analysis of biological structures. For optimal results, ultrathin and ordered sections are an absolute requirement. In this webinar you will get tips and tricks to optimize the workflow of your array tomography: - Fast and precise trimming of the sample block-face - Adhesion of a single section to create ribbons - Automated serial sectioning with the ARTOS 3D ultramicrotome - Acquisition and proce...
Expanding the limits of EM sample prep with ICE
November 20, 2018 17:45 - 57 minutes - 39.5 MBIn this webinar you will learn: - Improved vitrification: Specimen vitrification without synchronisation fluid - Vitrification strategies: Optimized freezing for different techniques - Light and electrical stimulation: Dissect cellular processes with millisecond precision - A look into the future: freezing of crystals Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular proc...
Ep 52 — Expanding the limits of EM sample prep with ICE
November 20, 2018 05:00 - 57 minutes - 39.5 MBIn this webinar you will learn: - Improved vitrification: Specimen vitrification without synchronisation fluid - Vitrification strategies: Optimized freezing for different techniques - Light and electrical stimulation: Dissect cellular processes with millisecond precision - A look into the future: freezing of crystals Plunge freezing and cryo imaging of proteins and complexes have revealed new details in understanding the machinery of the cell and how molecules are involved in cellular proc...
Using CRISPR/Cas9 to detect DNA sequences with AFM
November 13, 2018 17:15 - 37 minutes - 25.9 MBJoin Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging. In this webinar, you will learn: 1. How CRISPR/Cas9 can be used to "flag" alterations and mutations in DNA, rather than cut it 2. How pairing atomic force microscopy (AFM) with optical equipment found in DVD players can be used to map DNA at a faster rate than traditional DNA sequencing 3. Applications of ...
Ep 51 — Using CRISPR/Cas9 to detect DNA sequences with AFM
November 13, 2018 05:00 - 37 minutes - 25.9 MBJoin Dr. Jason Reed as he describes a novel method by which endonuclease-inhibited Cas9 can be employed as a programmable biomarker in high-speed atomic force microscopy (HS-AFM) imaging. In this webinar, you will learn: 1. How CRISPR/Cas9 can be used to "flag" alterations and mutations in DNA, rather than cut it 2. How pairing atomic force microscopy (AFM) with optical equipment found in DVD players can be used to map DNA at a faster rate than traditional DNA sequencing 3. Applications of ...
How to Use CRISPR to Accelerate Cancer Therapies
November 01, 2018 17:30 - 1 hour - 48.2 MBJoin Theo Roth as he describes his lab's novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human T cells, and permits individual or multiplexed modification of endogenous genes. Importantly, avoiding the use of viral vectors will result in accelerated research and clinical applications, reduce experimenta...
Ep 50 — How to Use CRISPR to Accelerate Cancer Therapies
November 01, 2018 05:00 - 1 hour - 48.2 MBJoin Theo Roth as he describes his lab's novel CRISPR-Cas9 genome-targeting system that does not require viral vectors to modify T cell genomes, but instead focuses on HDR. This allows rapid and efficient insertion of large DNA sequences at specific sites in the genomes of primary human T cells, and permits individual or multiplexed modification of endogenous genes. Importantly, avoiding the use of viral vectors will result in accelerated research and clinical applications, reduce experimenta...
Nucleic acids 101: Confirming Their Quality
October 16, 2018 16:45 - 51 minutes - 35.4 MBJoin us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn: - How to choose the best method to extract your nucleic acids - Which method you should chose to determine nucleic acid quality - How to avoid common pitfalls for both extraction and quality control Virtually all experiments in the molecular biology lab require high quality, pure nucleic acids as a starting material. This sounds simple enough. However, as a...
Ep 49 — Nucleic acids 101: Confirming Their Quality
October 16, 2018 05:00 - 51 minutes - 35.4 MBJoin us in this webinar as Dr. Victoria Doronina helps you determine the quality of your nucleic acids. In this webinar you will learn: - How to choose the best method to extract your nucleic acids - Which method you should chose to determine nucleic acid quality - How to avoid common pitfalls for both extraction and quality control Virtually all experiments in the molecular biology lab require high quality, pure nucleic acids as a starting material. This sounds simple enough. However, as a...
Live Cell Isolation by Laser Microdissection
October 10, 2018 17:45 - 51 minutes - 35.5 MBIn this webinar, you will learn: - How to best prepare your specimen for live cell isolation by laser microdissection - How to optimize your laser microdissection workflow - How to avoid common pitfalls of this technique Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other ...
Ep 48 — Live Cell Isolation by Laser Microdissection
October 10, 2018 05:00 - 51 minutes - 35.5 MBIn this webinar, you will learn: - How to best prepare your specimen for live cell isolation by laser microdissection - How to optimize your laser microdissection workflow - How to avoid common pitfalls of this technique Laser microdissection is a tool for the isolation of homogenous cell populations from their native niches in tissues to downstream molecular assays. Beside its routine use for fixed tissue sections, laser microdissection may be applied for live cell isolation. Unlike other ...
Reliable quantification of fluorescence images
October 09, 2018 16:45 - 38 minutes - 26.5 MBIn this webinar, you will learn: - How to overcome issues with stage drift and crosstalk between channels - How to obtain reliable images and reproducible quantification results - How to proceed with advanced image analysis after restoring images During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimen...
Ep 47 — Reliable quantification of fluorescence images
October 09, 2018 05:00 - 38 minutes - 26.5 MBIn this webinar, you will learn: - How to overcome issues with stage drift and crosstalk between channels - How to obtain reliable images and reproducible quantification results - How to proceed with advanced image analysis after restoring images During fluorescence image acquisition, many experimental uncertainties are introduced that affect the correct object interpretation and analysis. The blurring and the noise implicit in the image formation are two of the largest sources of experimen...
Crash Course: Developing a Fool-Proof ELISA
September 27, 2018 17:00 - 48 minutes - 33.1 MBJoin us in this webinar featuring Dr. Omonse Talton who will guide you through developing a "fool proof" enzyme-linked immunosorbent assay (ELISA). In this webinar, you will learn: - When and why you should use the different types of ELISAs– direct, indirect, sandwich, competitive/inhibition ELISA - Major considerations for developing your ELISA - Tried and tested tips for you to perform a successful ELISA The ELISA is one of (if not the most) common techniques in biology and biochemistry l...
Discovering PARP inhibitor resistance mechanisms with CRISPR
September 20, 2018 17:30 - 47 minutes - 32.4 MBIn this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talazoparib (BMN 673). The screen yielded one particularly interesting point mutation in the PARP1 gene. This mutation disrupted the ability of PARP1 to...
Ep 45 — Discovering PARP inhibitor resistance with CRISPR
September 20, 2018 05:00 - 47 minutes - 32.4 MBIn this webinar, Dr. Stephen Pettitt explains how he applies genome-wide targeted mutagenesis screens to elucidate the genetic basis of drug resistance. Using mouse and breast cancer cell lines, Dr. Pettitt’s team developed a targeted, genome-wide mutagenesis screen to identify mutations responsible for resistance to the potent PARP inhibitor talazoparib (BMN 673). The screen yielded one particularly interesting point mutation in the PARP1 gene. This mutation disrupted the ability of PARP1 to...
14 Ways to Measure Immune Cell Activation
September 18, 2018 16:45 - 1 hour - 45.4 MBActivation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease. There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity. In this webinar, we'll dis...
Ep 44 — 14 Ways to Measure Immune Cell Activation
September 18, 2018 05:00 - 1 hour - 45.4 MBActivation of immune cells is the all-important first step in mounting an immune response. Immune cell activation is a popular area of research because so much happens that is key to the downstream goal of fighting infection, cancer, and disease. There are many ways to measure immune cell activation, and they all have utility. Methods can be grouped into four main categories: Proliferation Assays, Cytokine Measurement, Surface Antigen Expression, and Cytotoxicity. In this webinar, we'll dis...
A Guide to Target Gene Validation Using Quantitative RT-PCR
September 12, 2018 16:45 - 1 hour - 47.8 MBWhile next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn: • Low (1-5 genes) vs medium (~300 genes) throughput experimental design • Pros and cons of self-designed vs “off the shelf” assays • How to set up your wet lab experiments start to finish • Downloadable example ste...
Ep 43 — Guide to Target Gene Validation With Quantitative RT-PCR
September 12, 2018 05:00 - 1 hour - 47.8 MBWhile next generation sequencing enables researchers to unveil expression levels of the entire genome, qRT-PCR remains the gold standard for measuring transcript levels of individual genes for functional studies and for the purposes of publication. In this webinar, you will learn: • Low (1-5 genes) vs medium (~300 genes) throughput experimental design • Pros and cons of self-designed vs “off the shelf” assays • How to set up your wet lab experiments start to finish • Downloadable example ste...
How to Ensure Your Cell-Based Assays Are Reproducible
September 05, 2018 18:30 - 45 minutes - 31.1 MBJoin us in this webinar featuring Dr. Vicki Doronina as she takes you through vital components of assay design. In this webinar you will learn: How to choose between immortalized cells and primary cells for your assay How to avoid sources of bias in your cell-based assays How to use high throughput assays, so you can achieve greater reproducibility By now, you have heard about the reproducibility crisis—the inability of scientists to reproduce experimental results. This crisis spurred journ...